Nettetidentify replicating cells and to sort cells based on their DNA content. A combination of Hoechst 33342 and propidium iodide have been extensively used for simultaneous flow cytometric and fluorescence imaging analysis of the stages of … NettetHoechst 33342 (from Sigma) comes as a lyophilized powder and is re-suspended at 1 mg/mL in water, filter sterilized, and frozen in small aliquots.. HBSS+ (Hanks Balanced …

Quantification of Apoptosis and Necrosis by Flow Cytometry

NettetHoechst 33342 and propidium iodide are frequently used together for simultaneous flow cytometric and fluorescence imaging analysis of the stages of apoptosis and cell-cycle distribution. For Research Use Only. Not for use in diagnostic procedures. … Hoechst 33342 and propidium iodide are frequently used together for … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes SYTO dyes are live cell, cell-permeant nucleic acid stains that exhibit bright … The Thermo Scientific FITC Antibody Labeling Kit is a multi-component kit … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes NettetThe rare population of CML stem cells can be identified by staining with cell surface markers, and then DNA-binding dyes Hoechst 33342 and propidium iodide (PI) are added to stain the DNA content which is changed when cells go through different phases of the cell cycle. Samples are run through the flow cytometer to be analyzed based on ... naughty christmas sweaters for sale

Cell Cycle Analysis of CML Stem Cells Using Hoechst 33342 and …

NettetPropidium iodide is a membrane-impermeable fluorescent DNA stain. It is used to stain apoptotic cells for flow cytometry, in situ hybridization, and immunohistochemistry … NettetThe apoptosis of GBM cells was analyzed by nuclear staining with Hoechst 33,258 (Invitrogen), or Annexin V/propidium iodide (Invitrogen) followed by flow cytometry as described previously. 18 For the nuclear Hoechst 33,258 staining, the treated GBM cells were stained with Hoechst 33,258 (3.7% formaldehyde, 0.5% Nonidet P-40, and 10 … NettetWhen choosing which dye to use, consider whether the dye may cover the markers of transfection. For example, Hoechst dye, ethidium bromide (EB), and propidium iodide (PI) are compatible for GFP. Hoechst dye, however, can be used only with a light X-Gal staining, since a dark X-Gal staining will cover the fluorescence of Hoechst dye. marius thorsrud