Tetramer stains usually analyze cytotoxic T lymphocyte (CTL) populations. CTLs are also called CD8+ T-cells, because they have CD8 co-receptors that bind to MHC class I molecules. Most cells in the body express MHC class I molecules, which are responsible for processing intracellular antigens and presenting at the cell's surface. If the peptides being presented by MHC class I molecules are f… WebbAdd 50 µL cell suspension into each test tube (e.g. 1 x 10 6 cells per tube). Incubate for 5 minutes at room temperature. Add 10 µL of T-Select MHC Tetramer and vortex gently. Incubate for 30-60 minutes at 2-8°C protected from light. Add any additional antibodies (e.g. anti-CD8) and vortex gently. Incubate for 30 minutes at 2-8°C protected ...
Production Protocols NIH Tetramer Core Facility
Webb15 sep. 2013 · While further studies will be needed to systematically examine the role JNK-p53-cross-talk in regulation of AICD in MHC class I restricted CD4 and CD8 T cells, taken together, our findings offer novel insights towards the biology of MHC class I restricted CD4 T cells by showing that the AICD in antigen experienced TCReng CD4 T cells is a … Webb2 juli 2001 · MHC class I tertrameric complex technology has been used to study immune responses to tumours and viral infections, and to probe the mechanism underlying autoimmnune disease. Tetramers have been used to stain peripheral blood lymphocytes and other cell types [ 7 , 8 ], cell suspensions made from tissue samples, and tissue … gem mint 10 shadowless charizard
Peptide-MHC class I and class II tetramers: From flow to mass
WebbMHC Tetramer. Creative Biolabs is a world leading biotechnological company in research and development of agents in the realm of immunology. With years of experience, we promise to offer best-in-class immunological reagent to meet the need of our customers. MHC Tetramers have emerged as an important tool for characterization of the … WebbMajor histocompatibility complex (MHC) class I molecules present peptide ligands on the cell surface for recognition by appropriate cytotoxic T cells. ... Figure 7 : MHC class I tetramer formation. Figure 8 : ELISA setup and results. Figure 9 : Typical HPLC profile of an MHC PE-streptavidin titration. Figure 10 : Flow cytometry. WebbTo address the role of CD8/MHC-I interactions, we generated tetramers composed of H2-K(b) molecules with mutations in the alpha 3 domain of H2-K(b) that abolish CD8 … gem mint 10 shiny charizard