Phis2.1

Author: tfsl

August undefined, 2024

Webb18 okt. 2024 · pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit (#K1617-1). Webb1 dec. 2024 · d Growth of yeast cells on SD/-Trp-Leu-His supplemented with 100 mM 3-AT. pHIS2.1-ProGA2ox3 plus pGADT7 and pHIS2.1-ProGA2ox7 plus pGADT7 served as the negative controls.

pHIS2 [PVT4037] - $162.00

Webb1 sep. 2024 · 1-Methylcyclopropene counteracts ethylene promotion of fruit softening and roles of MiERF2/8 and MiPG in postharvest mangoes. ... and pHIS2-MiPG were cultivat ed on SD/-T r p/-His for 3 days, and . Webb16 feb. 2024 · To explore the involvement of bZIP genes in flower senescence, one up-regulated transcript encoding a putative ocs element binding factor, namely PhOBF1 (SGN accession no. Peaxi162Scf00285g00011), was identified during petunia flower senescence through transcriptome sequencing analysis [ 36 ]. cully 39820

Disruption of the bHLH transcription factor Abnormal …

http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html WebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & Screening Kit (#K1617-1). Webb1 mars 2024 · MdGSTF6-p-pHIS2 vectors were first grown on −Trp/−His medium containing 3-amino-1,2,4-triazole (3-AT) for screening. The concentration of 3-AT used was 100 mM. east hanover marriott hotel

Matchmaker One-Hybrid Library Construction & Screening Kit

Phis2.1

Setaria italica SiWRKY89 enhances drought tolerance in …

Webb1 juni 2024 · When the insertion sequence is predicted as known cis-acting element , construct the pHIS2 reporter by three tandem copies of the studied elements inserted at …

Did you know?

Webbyeast reporter vector phis2 1 bd ( TaKaRa ) 95. TaKaRa yeast reporter vector phis2 1 bd. Yeast Reporter Vector Phis2 1 Bd, supplied by TaKaRa, used in various techniques. Bioz … Webb17 juni 2024 · Expression pattern analysis of OsbZIP62 under abiotic stresses and hormones. a, Relative expression level of OsbZIP62 under various stresses, including PEG6000 (15%), cold (4 °C), heat (42 °C), NaCl (150 mM), H 2 O 2 (1%) and ABA (100 μM). b, Main stress-related cis-acting elements in the OsbZIP62 promoter region.c, Relative …

WebbPlasmid pHIS2-hsSTAM1 (1-143) from Dr. James Hurley's lab contains the insert phis2- STAM1(1-143) and is published in Structure. 2009 Mar 11. 17(3):406-16. This plasmid is … WebbThe promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs ...

Webb12 okt. 2024 · Moreover, a yeast one-hybrid assay was performed to verify the interaction between CnGATA20 and the CnOLE18 promoter. pHIS2.1-pro53 and pGADT7-53 (pHIS2.1-pro53+pGADT7-53) were cotransferred into yeast Y187 as a positive control, and pHIS2.1-proCnOLE18+pGADT7-53 was used as a negative control. WebbThe target DNA sequence, or Bait Sequence, is cloned into pHIS2.1 as one copy or tandem repeats. Interaction between a DNA-binding protein and the target sequence stimulates …

Webb14 jan. 2015 · The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs ...

Webb24 mars 2024 · In case of 4CRM1-pHis2.1, 4CRM1mut2-pHis2.1, and 4CRM1mut5-pHis2.1, 50 mM 3-amino-1,2,4-triazole (3-AT) was sufficient to suppress background growth of yeast colonies. DNA from the TF-only prey library in pDEST_GAD424 (Mitsuda et al. 2010) was transformed into Y1HGold containing 4CRM1-pHis2.1. east hanover movies cinema 12WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … cully 40340jWebbClose You have no items in your shopping cart.. Home; Plasmid; p53- His2 Model: PVT11251 east hanover locksmithWebb1 dec. 2024 · According to the hereditary mode of sterility genes, this vital agronomic trait can be further divided into cytoplasmic male sterility (CMS) and genic male sterility … cully 39921Webb25 okt. 2016 · The 43-bp NWRE fragment amplified by PCR was inserted into pHIS2.1 (Clontech, CA, USA) to generate the plasmid pHIS2.1-NWRE as bait. The coding regions of IbbHLH3 and IbbHLH4 were separately cloned into pGADT7 as effectors. Lithium acetate-mediated transformation was performed to transform the constructs into yeast strain … cully 39916jWebbpHis2: 质粒类型: 酵母单杂交载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点，限制性内切酶: 载体大小: 7207bp: 5' 测序引物及序列:--3' 测序引物及序列:--载体标签: TRP1, HIS3: … cully 3/8 hollow wall anchorsWebb27 mars 2015 · In case of pHis2.1-Seq15 containing 4CRM-DJ1E, 50 m m 3-AT was sufficient to suppress background growth. As prey to select for TFs that bind to the … cully 50133j