Polyhedrin promoter
Web1 Introduction Overview Introduction pBlueBac4.5/V5-His is a baculovirus transfer vector used for expression of recombinant proteins in lepidopteran insect cell lines. It utilizes the polyhedrin promoter from Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) for high-level expression of the gene of interest (Crawford and WebModified polyhedrin promoter (Ppolh) was designed by repeating burst sequences (BSs) and adopted to overexpress rat α2,6-sialyltransferase (ST6Gal I) in silkworm. Modified Ppolh of five BSs with VLF-1 coexpression yielded 2.9U/ml ST6Gal I activity and 32.5mU/mg specific activity, which was 1.7- and 2.3-fold higher, respectively compared to Ppolh.
Polyhedrin promoter
Did you know?
WebThe recombinant baculovirus was constructed to carry an enhanced green fluorescent protein under the control of polyhedrin promoter as a fluorescent selection marker in the Sf21 cell line. Recombinant baculoviruses were then used to transduce S2 cells with target gene expression cassettes containing a Drosophila heat shock protein 70, an actin 5C, or … WebHigh level target protein expression controlled by of polyhedrin promoter GFP marker to simplify manipulations Compatible with Sf9, Sf21, Hi-Five insect cells and with wide range of transfection reagents and media recommended for baculovirus work . 1. ProGreen™ GFP + baculovirus vector DNA, 25 μ l, 2.5 μ g 2.
WebWe noticed that marine mussel has silk-like domains containing many repeats with abundance of glycine and alanine. In the present work, we redesigned mussel-derived silk-like gene sequence which contains alternating repeated and nonrepeated regions with optimized codons for Escherichia coli. For successful expression of recombinant mussel …
WebType IB DNA topoisomerases are enzymes to change the topological state of DNA molecules and are essential in studying replication, transcription, and recombination of nucleic acids in vitro. WebDec 5, 2016 · Rep78 is under the control of a truncated promoter for the immediate-early 1 gene of Orgyia pseudotsugata nuclear polyhedrosis virus (DIE1) and the expression of Rep52 is under control of the AcNPV polyhedrin (polh) promoter. BacCap expresses capsid proteins VP1, VP2 and VP3 under the transcriptional control of the polyhedrin promoter.
WebHypertranscription and temporal expression from theAutographa californica nuclear polyhedrosis (AcNPV) baculovirus polyhedrin promoter involves an α-amanitin-resistant RNA polymerase and requires a trans-acting viral factor(s). We previously reported that a 30-kDa host factor, polyhedrin promoter binding protein (PPBP), binds with unusual affinity, …
WebThe Bac-to-Bac Baculovirus Expression System exists an efficient method for producing recombinant baculovirus for expression testing in insect cells. Of Bac-to-Bac System relies on generation of high-technology baculovirus by site-specific transpo first alert brk sc9120ff vs sc9120bWebThe baculovirus homologous region (hr1) sequence has been disclosed as a transcriptional enhancer of foreign gene expression. An additional copy of the hr1 element contained within a recombinant baculovirus has been demonstrated to be capable of further enhancing expression of foreign genes placed under the transcriptional control of a promoter such … european sailing championshipWebMay 6, 2012 · Background: The polyhedrin gene promoter has an essential role in regulating foreign gene expression in baculovirus expression vector systems (BEVS); however, the … first alert brk sc9120lbl hardwiredWebMay 6, 2012 · The polyhedrin gene promoter has an essential role in regulating foreign gene expression in baculovirus expression vector systems (BEVS); however, the high-level … european sail tower sps conceptWebJan 21, 2024 · PH (1–110) peptide fused to GFP form particles. We developed a universal system to produce fusion proteins using as template the first 110 amino acids from AcMNPV polyhedrin protein. A transfer plasmid containing the strong polyhedrin promoter drives the expression of the PH (1–110) followed by a poly-linker [], which allows the … first alert brk sc9120b reviewWebDec 23, 1987 · The polyhedrin gene promoter of the Autographa californica nuclear polyhedrosis virus was analysed with respect to which sequences are required upstream … european salad company limitedWebTo demonstrate that binding of PPBP to its cognate sequences, essentially the octa- and hexamotifs within the initiator promoter, is critical for transcription in vivo, three sets of 65 … first alert brk sc9120b